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Biodiagnostics-Group

The Cellular Biodiagnostics group develops cellular test systems capable of examining the impact of space conditions on humans already on cellular and molecular level. Bioassays to be developed relay on the receptor-reporter principle well known for many bacterial assays. The sensing systems consist of specially designed plasmids or vectors, where promoters, which is dependent on certain environmental conditions, act as a sensing receptor. Fluorescent proteins are valuable tools for reporter components. Receptor-reporter assays allow the detection of cellular signal transduction events dependent on special environmental parameters.

Rodent cells for biological dosimetry

Mammalian cell culture research has been progressively helpful in investigating the influence of different environmental conditions on viability, productivity and cytogenetic effects. From the obtained results risk was extrapolated to man. For testing toxicity testing a number of methods have been developed which base on either the viability of cell populations or on the proliferative capacity of single cells.

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Bacterial tests for toxicity determination

The bacterial Lux-Fluoro test measures the genotoxic (SOS-Lux-Test) and cytotoxic (Lac-Fluoro-Test) potency of agents. The SOS-Lux test is based on the bacterial SOS-response which is induced in presence of DNA-damaging agents. It uses the strong SOS-dependent ColD-promoter as receptor component and the promoterless luxCDABFE genes of Photobacterium leiognathi as reporter component. With this system dose-dependent light yields can be recorded in Salmonella typhimurium treated with genotoxic agents.

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	Mammalian cell bioassays for the international space station In space, man encounters many strange and potentially harmful environmental factors. Cosmic radiation and microgravity are limiting for long duration space flights. Humans and whole animals' responses to microgravity have already been studied in depth.
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Head

Dr.rer.nat. Christa Baumstark-Khan
German Aerospace Center
Institute of Aerospace Medicine, Radiation Biology
Tel.: +49 2203 601-3140
Fax: +49 2203 619 70


Matroshka


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Biodiagnostics-Group

Rodent cells for biological dosimetry

Bacterial tests for toxicity determination

Mammalian cell bioassays for the international space station