In 2021, the second campaign was conducted in the EDEN ISS greenhouse in Antarctica. From January 2021 to January 2022, the greenhouse was successfully operated by NASA guest scientist Jess Bunchek. The Astrobiology group focused on microbial quantity and diversity in surface samples from the greenhouse and in liquid samples from plant nutrient tanks during the second campaign. In addition, the efficiency of cleaning procedures was examined from a microbial perspective. In April 2022, all samples (surface swab samples and liquid samples) arrived at DLR Cologne. A first set of samples has been evaluated in the meantime and corresponds to the data of the first campaign. The evaluation of the remaining samples is still ongoing.